Fig. 2

SerpinA3N was highly expressed in TLE mice and mainly expressed in hippocampal astrocytes. A The expression of SerpinA3N was determined by qPCR in the hippocampus of epileptic mice at 1, 10, and 35 d after KA injection (n = 4). Normalized to the sham level. Gapdh served as the internal control. B, D Western blotting and densitometric quantitative analysis of SerpinA3N in the hippocampus of epileptic mice at 1, 10, and 35 d after KA injection (n = 3). Normalized to the sham level. GAPDH served as the internal control. C, E Western blotting and densitometric quantitative analysis of SerpinA3 in the temporal cortex of autopsy patients and TBI and TLE patients (n = 3). Normalized to autopsy patients’ level. GAPDH served as the internal control. F, H Immunostaining of SerpinA3N with the cell markers GFAP, Iba1, NeuN, and Olig2 in the hippocampal CA3 region of epileptic mice at 1 day after KA injection and the hippocampus of TLE patients. Scale bar = 50 μm. G, I Percentage of cells that are double-positive for SerpinA3N and GFAP/Iba1/NeuN or Olig2 among the total cells that are positive for GFAP/Iba1/NeuN or Olig2. All data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. KA: kainic acid; TBI: traumatic brain injury; TLE: temporal lobe epilepsy