Fig. 4

SerpinA3N knockdown alleviates KA-induced neuroinflammation and decreases the number of hippocampal astrocytes and microglia in vivo. A The mRNA levels of Tnf-α, Il-1β, Il-18, Il-6, and Nf-κb in the hippocampus of SerpinA3N knockdown mice at 35 d post-SE, as determined by qPCR (n = 4). Normalized to the Scr + veh level. GAPDH served as the internal control. B, C Western blotting and densitometric quantitative analysis of TNF-a, IL-1β and IL-18 in the hippocampus of SerpinA3N knockdown mice at 35 d post-SE (n = 4). Normalized to the Scr + veh level. GAPDH served as the internal control. D, E Representative fluorescence micrographs of GFAP and IBA1 expression in the hippocampal CA3 area of SerpinA3N knockdown mice with or without KA treatment for 35 days. Scale bar = 50 μm. F, G The panels show the cell counts of cells positive for GFAP and IBA1 (n = 3). All data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. SE: status epilepticus; Scr: scrambled AAV vectors; veh: vehicle; KA: kainic acid; ShRNA: AAV vectors containing short hairpin RNA targeting SerpinA3N