Fig. 5

SerpinA3N aggravates seizures and KA-induced hippocampal neuronal loss in vivo. A, B SRS number and the mean daily frequency of the epileptic mice with or without SerpinA3N knockdown from 28 to 35 d post-SE by video monitoring (n = 6). C, D SRS number and the mean daily frequency of the epileptic rats with or without overexpression of SerpinA3N from 28 to 35 d post-SE by video monitoring (n = 6). E, G Representative Nissl staining photomicrographs of the ipsilateral hippocampus in response to AAV vectors and/or KA injection from SerpinA3N-overexpressing and SerpinA3N knockdown mice with or without KA treatment for 35 days (n = 3–4). F, H The panels show the counts of cells in the CA1, CA3, and DG regions of the hippocampus ipsilateral to the AAV vectors and/or KA injection side (n = 3–4). All data are shown as the mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, ***p < 0.001. SRS: spontaneous recurrent seizures; SE: status epilepticus; AAV: adeno-associated virus; KA: kainic acid; HIP: hippocampus