Fig. 6

SerpinA3N activates the NF-κB signaling pathway in mice with TLE. A Heatmap with clustering of differentially expressed genes (top 1000) in the hippocampus of mice with SerpinA3N overexpression at 14 d after AAV vector injection. The color scale shows genes that are upregulated (red) or downregulated (blue) relative to the mean expression of all samples (n = 3). B The numbers of differentially expressed genes. C KEGG pathway enrichment analysis (ranked by p value). Dot size represents the number of genes in each KEGG pathway. The gene ratio (x-axis) is the percentage of significant genes over the total genes in a given pathway. D GSEA of differentially expressed genes. In each enrichment plot, the green curve corresponds to the ES curve, which is the running sum of the weighted ES. The FDR estimates the statistical significance of a single gene set's enrichment score. E–H Western blotting and densitometric quantitative analysis of NF-κB (p65), p-NF-κB (p65), p-IKKα + β, and IKKα + β in response to AAV vectors and/or KA injection from SerpinA3N-overexpressing and SerpinA3N knockdown mice with or without KA treatment for 35 days. GAPDH served as the internal control (n = 4). All data are shown as the mean ± SEM. Ns represents no significance. *p < 0.05, **p < 0.01, ***p < 0.001. AAV: adeno-associated virus; KEGG: Kyoto Encyclopedia of Genes and Genomes; GSEA: gene set enrichment analysis; ES: enrichment score; KA: kainic acid