Fig. 7

SerpinA3N interacts with RYR2 and promotes its protein levels. A PPI network constructed by the HitPredict website. B Detection of the interaction between SerpinA3N and RYR2 by Co-IP. C Representative fluorescence micrographs of RYR2, SerpinA3N, and GFAP expression in the hippocampal CA3 region of mice at 1 day after KA injection (scale bar (left) = 50 μm. Scale bar (right) = 20 μm). D, E Western blotting and densitometric quantitative analysis for RYR2 and p-RYR2 (S2808) in the hippocampus of mice at 35 d after KA injection. GAPDH served as the internal control (n = 3). F, G Western blotting and densitometric quantitative analysis of RYR2 and p-RYR2 (S2808) in the hippocampus of the SerpinA3N-overexpressing mice at 7 d post-SE. GAPDH served as the internal control (n = 3). H, I Dual immunofluorescence analysis with anti-RYR2 (red), anti-p-RYR2 (red) and anti-GFAP antibodies (green) in hippocampal astrocytes of sham and epileptic mice at 35 d after KA injection. (Scale bar = 50 μm; n = 3). All data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus the sham group. PPI: protein–protein interaction; Co-IP: co-immunoprecipitation