Fig. 6

GSK-343 administration modulated neuroinflammation after MPTP injection. Western blot analysis revealed an increase of EZH2 and TRAF6 expression in MPTP-injected mice, compared to the Sham mice however, GSK-343 (1 mg/kg, 5 mg/kg and 10 mg/kg) administration was able to reduce their levels (A, B, densitometric analysis A1–B1a). MPTP-injected mice showed a decrease expression of IĸB-α, compared to the Sham mice (C, densitometric analysis C1). NF-kB and NIK levels resulted elevated in the MPTP-intoxicated mice compared to Sham animals (D, E densitometric analysis D1–E1). GSK-343 (1 mg/kg, 5 mg/kg and 10 mg/kg) administration increased IKB-α levels and at the same time reduced NF-kB and NIK expression. Data are representative of at least three independent experiments. Values are means ± SEM. One-way ANOVA test. ***p < 0.001 vs Sham; ^#p < 0.05 vs MPTP; ^###p < 0.001 vs MPTP