Fig. 8

Immunofluorescence analysis of Iba1 and GFAP after MPTP intoxication. Tissues sections were stained with antibodies against Iba1 (green) or GFAP (green) and DAPI to highlight cell nuclei (blue). MPTP-injected mice showed high number of GFAP + cells (B, score F) and IBA-1 + cells (H, score N), compared to the Sham groups (A, score F; G, score N). Number of GFAP and IBA-1 + cells decreased after GSK-343 treatment at the dose of 1 mg/kg (C, score F; I, score N) more effectively at the highest dose of 5 mg/kg and 10 mg/kg (D, E, score F; L–M, score N). Data are representative of at least three independent experiments. Values are means ± SEM. One-way ANOVA test. ***p < 0.001 vs Sham; ^##p < 0.01 vs MPTP; ^###p < 0.001 vs MPTP