Fig. 1
ATP-P2X7R activation is an important mechanism to induce retinal microglia senescence. A ATP concentrations in the aqueous humor in different groups. ***P < 0.001 vs. Young ARC group. B The expression of P2X7R in retina was analyzed from GSE26299 dataset. ***P < 0.001 vs. control group. C Functional enrichment indicated differential expressed genes between high and low P2X7R expression group. D Measurement of IOP in COH model mice. *P < 0.05 vs. Young 0 d, &P < 0.05 vs. Old 0 d. E Contrast sensitivity in the COH model of young mices. ****P < 0.0001 vs. 0 d. F, G FG was injected into the superior bilateral colliculi at 7 days before COH injury injection. Fluorescence microscopy analysis of flat-mounted retinas and FG-labeled cells was performed at 6 weeks after COH injury. The remaining fluorochrome-labeled cells were quantified using image analysis and expressed as the mean number of cells 6 SEM (n = 5 per group, **P < 0.01 vs. Young COH). Scale bar: 50 μm. H–L Representative photomicrographs of Iba1, γ-H2AX and P53 immunofluorescence staining in the retinal extract from mice. **P < 0.01 vs. young sham. Scale bar: 50 μm. M, N The phagcytosis of microglia from adult mice retina. **P < 0.01 vs. young sham. Scale bar: 50 μm. GCL ganglion cell layer, IPL inner plexiform layer, INL inner core layer, OPL outer plexiform layer, ONL outer nuclear layer