Fig. 2

Effects of BzATP stimulation on microglial senescence in time- and dose-dependent manner. A The senescence of microglia was detected by SA-β-Gal. Scale bar: 50 μm. B Percentage of β-gal stained cells. *P < 0.05 vs. Control group. C γ-H2AX in microglia was detected by immunofluorescence assay. Scale bar: 50 μm. D Percentage of γ-H2AX stained cells. *P < 0.05 vs. Control group. E–G Western blot analysis of age-related markers under different concentrations of BzATP treatment. H–J Expression of E–G protein was evaluated by ImageJ. Protein expression levels were normalized according to GAPDH expression levels. Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. Control group