Fig. 3
BzATP-P2X7R pathways specific activation of microglia mitophagy. Microglia were stimulated with 50 μM BzATP for 0-48 h. A, B Mitochondrial membrane potential was determined by JC-1 staining. The red fluorescence of the JC-1 probe indicates healthy mitochondria, while the green fluorescence of the JC-1 probe indicates damaged mitochondrial potential. ***P < 0.001 vs. Control group. C Representative images of PINK1 and LC3B immunofluorescence staining in control and BzATP-treated group. Scale bar: 25 μm. D Western blot analysis was performed to assess mitophagy-related protein levels after BzATP stimulation. E The expression of (D) protein was evaluated by ImageJ. Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. Control group