Fig. 4
Activation of Mitophagy reverses microglial senescence. Microglia were incubated with BzATP (50 μM) combined with CCCP (5 μM) and A438079 (50 μM) treatment, respectively. A The protein expressions of PINK1, Parkin, LC3B and TIM23 were quantified, and GAPDH was used as the loading control. B–E Expression of A protein was evaluated by ImageJ. *P < 0.05 vs. Control group, &P < 0.05 vs. BzATP group. F Western blotting analysis of P53, P21 and P16 protein expression. GAPDH was used as the load control. G–I Expression of F protein was evaluated by ImageJ. *P < 0.05 vs. Control group, &P < 0.05 vs. BzATP group. J SA-β-Gal was used to detect the senescence of microglia. K Percentage of β-gal stained cells. Scale bar: 50 μm. L γ-H2AX in microglia was detected by immunofluorescence assay. M Percentage of γ-H2AX stained cells. Scale bar: 50 μm