Fig. 5

Effects of overexpression of PINK1 on mitophagy and senescence in microglia. Microglia treated with PINK1 OE and/or BzATP (50 μM, 12 h). A The senescence of microglia was detected by SA-β-Gal. Scale bar: 50 μm. B γ-H2AX in microglia was detected by immunofluorescence assay. Scale bar: 50 μm. C Western blotting was used to detect the protein expressions of P53, P21 and P16 after PINK1 overexpression in microglia, and GAPDH was used as the loading control. D–F Expression of C protein was evaluated by ImageJ, mean ± SD of three independent experiments. *P < 0.05 vs. Vector group, &P < 0.05 vs. BzATP + Vector group. G The expression of PINK1, Parkin, LC3B and TIM23 proteins was analyzed by Western blotting. GAPDH was used as the load control. H–K Expression of G protein was evaluated by ImageJ, mean ± SD of three independent experiments. *P < 0.05 vs. Vector group, ^&P < 0.05 vs. BzATP + Vector group