Fig. 6

Effect of microglia overexpression of PINK1 on survival rate of RGC. Microglia treated with PINK1 OE and/or BzATP (50 μM, 12 h). A–D Secretion of SASP by microglia under different treatments. The bar graph shows the changes in extracellular concentrations of MCP1, Il-6, TNF-α and GSF under different treatments. Mean ± SD of three independent experiments. *P < 0.05 vs. Vector group, ^&P < 0.05 vs. BzATP + Vector group. E Representative images of live (green) and dead (red) RGC after co-cultured with microglial supernatants treated with PINK1 OE and/or BzATP. Scale bar: 50 μm. F The bar graph summarizes the average ratio of live/dead cells under different conditions. *P < 0.05 vs. Glu, &P < 0.05 vs. Glu + MCM (BzATP + Vector). G, H The bar chart shows the changes in Bax and Bcl2 mRNA under different treatments. I–K The bar chart shows the changes in Bax and Bcl2 protein under different treatments. Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. Vector group, ^&P < 0.05 vs. BzATP + Vector group