Fig. 7

Transplantation of bone marrow cells from young mice effectively ameliorated RGC injury in COH model of aged mice. After 2 weeks of feeding on a diet formulated with 1200 ppm plx5622, microbeads were injected into the anterior chamber, and BMCs was injected into the tail vein. A Expression of Iba1 in retinal microglia. Scale bar: 50 μm. B Staining of BMCs retinas with glial labeled anti-IBA1 antibody (red) revealed three distinct labeled cell populations (red, green, and yellow appearance). Anti-iba1 antibody marks retinal microglia (red). GFP represents differentiated cells after the migration of bone marrow cells into the retina (green). Yellow round microglia indicate the differentiation of bone marrow cells into microglia (arrows). Scale bar: 50 μm. C, D FG-labeled RGC were analyzed by fluorescence microscopy after bone marrow transplantation. Scale bar: 50 μm. Each dataset is expressed as mean ± SD. *P < 0.05 vs. 0 d, ^&P < 0.05 vs. 14d. GCL ganglion cell layer, IPL inner plexiform layer, INL inner core layer, OPL outer plexiform layer, ONL outer nuclear layer