Fig. 3

Chronic LC activation from the onset of motor symptoms affects the cortical EAE-associated glial response. Representative images showing astrocyte activation in the A PL/IL and C M2/M1 cortices, and B, D the quantification of GFAP immunoreactivity (IR) in arbitrary units (AU). E-J Microglial activation reflected by Iba1 immunofluorescence (green) in the E PL, F IL, H M2 and I M1 cortices, and G, J the relative area of these brain regions stained for Iba1. The data represent the mean + SEM and each point corresponds to an individual mouse (n = 4–5 per group). ^##p < 0.01, ^###p < 0.001 EAE versus naïve (Additional file 1: Table S4). Scale bars: A, C, 200 µm; E, F, H, I, 100 µm