Fig. 1

Repeated FTY720 dose regimen reduces retinal inflammatory response to laser-induced injury. a–d Inflammatory response of untreated and FTY720-treated Cx3cr1^GFPCcr2^RFP mice. a Images show differences in resident and blood-borne immune cell recruitment on days 1 and 7 in the damaged site (delimited by magenta dashes). By day seven post-injury, untreated mice still exhibit clustered Cx3cr1⁺ cells and PL at the site of damage a week post-injury, while PL infiltration has reduced to baseline by day 7 in retinas treated with FTY720 show no significant PL infiltration and Cx3cr1⁺ cells are evenly distributed throughout the retinal parenchyma. Inserts of each picture show morphological stages of Cx3cr1⁺ cell activation in untreated and FTY720-treated mice. The arrow indicates the direction in which the damaged site is located. b, c Quantification of the number of GFP⁺ cells and RFP⁺ PL per lesion of untreated and FTY720-treated Cx3cr1^GFPCcr2^RFP retinas before injury (baseline) and at pre-defined time points (days 0, 1, 4, 7, 10 and 14). Significant differences (**p < 0.01 and ****p < 0.0001) between untreated and FTY720-treated mice were determined by using a post hoc Bonferroni one-way ANOVA test (n = 8). d Quantification of the polarization coefficient of untreated and FTY720-treated resident immune cells after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (****p < 0.0001) between untreated and FTY720-treated mice were determined by using a post hoc Bonferroni one-way ANOVA test (n = 8). For both groups, day 0 was chosen as calibrator [NRQ (normalized relative quantification) = 1]. e Quantification of the primary and terminal processes per cell after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (*p < 0.05) between untreated and FTY720-treated mice were determined by using a post hoc Bonferroni one-way ANOVA test (n = 8). Field of view is ≈425 µm