Fig. 4

Genetic depletion of lymphocytes accelerated vascular recovery compared to broad PL reduction. a, b Kinetics of retinal injury detected in reflectance in untreated, FTY720-treated and Rag1 KO mice. Untreated and FTY720-treated mice are the same cohorts shown in Fig. 2. a Images show a reduction in the size of hypo- and hyper-reflectivity in the damaged site of FTY720-treated and Rag1 KO retinas on day 4 unlike untreated retinas. b Quantification of the damaged area of untreated and FTY720-treated, and Rag1 KO retinas after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (**p < 0.01 and ****p < 0.0001) between untreated and FTY720-treated mice were determined by using a post hoc Bonferroni two-way ANOVA test (n = 8). c Representative fluorescein angiographs of untreated and FTY720-treated, and Rag1 KO eyes on day 1. d Quantification of dye that leaks deep in the retina, identified as leakage area, after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (**p < 0.01 and ****p < 0.0001) between untreated, FTY720-treated and Rag1 KO mice were determined by using a post hoc Bonferroni two-way ANOVA test (n = 8). For both groups, day 0 was chosen as calibrator [NRQ (normalized relative quantification) = 1]. Field of view is ≈425 µm