Fig. 5

Beneficial effect of CD8 depletion on resident immune cell activation and PL recruitment. a–d Inflammatory response of CD4- and CD8-treated Cx3cr1^GFPCcr2^RFP mice. a Images show differences resident and blood-borne immune cell recruitment on day 1 in the damaged site (delimited by magenta dashes). Anti-CD4 treatment lessened Cx3cr1⁺ cell recruitment to the damaged area. Inserts show morphological stages of Cx3cr1⁺ cell activation in anti-CD4- and CD8-treated mice. The arrow indicates the direction in which the damaged site is located. b, c Quantification of the number of GFP⁺ cells and RFP⁺ PL per lesion of anti-CD4- and CD8-treated Cx3cr1^GFPCcr2^RFP retinas before injury (baseline) and at pre-defined time points (days 0, 1, 4, 7, 10 and 14). Significant differences (*p < 0.1, **p < 0.01 and ****p < 0.0001) between anti-CD4- and CD8-treated mice were determined by using a post hoc Bonferroni two-way ANOVA test (n = 8). d Quantification of the polarization coefficient of anti-CD4- and CD8-treated resident immune cells after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (*p < 0.1, **p < 0.01 and ****p < 0.0001) between anti-CD4- and CD8-treated mice were determined by using a post hoc Bonferroni one-way ANOVA test (n = 8). For both groups, day 0 was chosen as calibrator [NRQ (normalized relative quantification) = 1]. e Quantification of the primary and terminal processes per cell after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (*p < 0. 1) between anti-CD4- and CD8-treated mice were determined by using a post hoc Bonferroni one-way ANOVA test (n = 8). Field of view is ≈425 µm