Fig. 6

Protective effect of CD8 depletion on lesion recovery and BRB regeneration. a, b Kinetics of retinal injury detected in reflectance in CD8- and CD4-depleted mice. a Images show the absence of hypo- and hyper-reflectivity in the damaged site of CD8-depleted retinas on day 4, whereas the injury was visible in CD4-depleted retinas (delimited by magenta dashes). b Quantification of the damaged area of anti-CD8- and CD4-treated retinas after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (****p < 0.0001) between two groups were determined by using a post hoc Bonferroni one-way ANOVA test (n = 8). c Representative fluorescein angiographs of eyes treated with anti-CD8- or CD4-antibody on day 1. The leakage area was observed only in fluorescein angiograms of anti-CD4-treated animals, while minimal vascular damage was detected in the depth of CD8-depleted retinas. d Quantification of fluorescein that deep in the retina, identified as leakage area, after injury (day 0) and at pre-defined time points (days 1, 4, 7, 10 and 14). Significant differences (**p < 0.01 and ****p < 0.0001) between anti-CD8- and CD4-treated mice were determined by using a post hoc Bonferroni one-way ANOVA test (n = 8). For both groups, day 0 was chosen as the calibrator [NRQ (normalized relative quantification) = 1]. Field of view is ≈425 µm