Fig. 9

Perforin deletion has the same beneficial effect as the depletion of CD8⁺ T cells. a, b) Kinetics of retinal injury detected in reflectance in CD8-treated and Prf1 KO mice. Anti-CD8-treated animals were part of the same cohorts in Fig. 6. a Images show similar area of hypo- and hyper-reflectivity in the damaged site of Prf1 KO and CD8-treated mice on day 1 (delimited by magenta dashes). b Quantification of the damaged area of CD8-treated and Prf1 KO retinas after injury (day 0) and at pre-defined time points (day 1, 4, 7, 10 and 14) (n = 8). c Representative fluorescein angiographs of CD8-treated and Prf1 KO eyes on day 1. A small accumulation was observed in fluorescein angiograms of Prf1 KO mice, as observed after anti-CD8-antibody treatment. d Quantification of dye that leaks in the depth of the retina, identified as leakage area, after injury (day 0) and at pre-defined time points (day 1, 4, 7, 10 and 14) (n = 8). For both groups, day 0 was chosen as calibrator [NRQ (normalized relative quantification) = 1]. Field of view is ≈425 µm