Fig. 6

The NLRP3 inhibitor MCC950 blocked mTBI-induced caspase-1-mediated inflammatory signals in the brain and hindpaws of mice in vivo. A Sample images show caspase-1 activation reporter bioluminescence signals in mouse brains and hindpaws at 1 and 3 days after the third injury. Mice were either injected with saline (vehicle) or MCC950 intraperitoneally at 10 mg/kg (5 mg/mL) at 1 h and 24 h after each injury. The analyzed ROIs were quantified across all time points. B–E A significant decrease in caspase-1 activity signals was detected in the brain (B, C) and paw (D, E) of MCC950-treated animals (1 and 3 days) compared to the vehicle-treated group (n = 10–15 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, repeated-measure ANOVA, Tukey’s HSD)