Fig. 1
Study design and exposure characterization. A 8-week-old female C57BL/6J mice were exposed to woodsmoke derived from woodchip biomass every other day for 2 weeks at an exposure concentration average ~ 500 µg/m3 per exposure. After exposure, mice were euthanized at serial timepoints of 1-, 3-, 7-, 14-, and 28-day post-exposure. B Particle size distribution percentile curve. Median particle size measured between 0.138 µm and 0.145 µm. C Exposure averaged 532 µg/m3 for total exposure duration and 0.044 mg/m3 for 24-h average. Red line: exposure average per 4 h exposure. Blue line: exposure average considering 24 h per day. D Biomass-derived carbon monoxide (CO) and oxides of nitrogen species (NOx) concentrations (both below USEPA standards). E Bronchoalveolar lavage fluid was extracted for cell staining and differential analyses. Total cells were statistically increased in the lung on day 3, but no other differences were seen for other cells or on other days. F No significant weight differences were seen between groups for the duration of experimentation