Fig. 2

Examining endothelial cell-specific responses by flow cytometry. A, B CD31 endothelial cells representing blood brain barrier; y-axis: marker grouping; x-axis: day post-exposure; colored radar plot slice: marker of interest measured; dotted line: 100% baseline control; all values normalized to filtered air (FA) controls. A Anti-inflammatory CD31^Hi cells showed an immediate increase in population percent and a decrease in iNOS (day 1). On day 3, population percent remained significantly increased coinciding with a reduction in CCL2 expression. Population percent trended higher on day 7 and was significantly higher on day 14. B Proinflammatory CD31^Med saw a decreased population percent, but increased TNFα, VCAM-1, and CCL2 expression through days 1–7. ICAM expression increased on day 3 and remained up until day 14