Fig. 3

Microglial activation and peripheral immune infiltration. A–C y-axis: cell subset grouping; x-axis: day post-exposure; colored radar plot slice: marker of interest measured; dotted line: 100% baseline control; all values normalized to filtered air (FA) controls. A Activated microglial population increased on day 28. TNFα increased on day 7 and remained increased on day 28. CD45 trended upward starting on day 7 through day 28. iNOS expression increased on day 28. B Nonspecific peripheral immune infiltration population percent trended upward on day 7, showed significant increases on day 14, and was resolved by day 28. TNFα expression increased on day 14 and trended upward on day 28. CCL2 expression trended upward starting on day 7 and remained trending upward on day 28. CD45 expression increased on days 7 and 14 but was resolved by day 28. MHCII trended upward on day 7 and showed significant increases on days 14–28. C Infiltrating polymorphonuclear neutrophils (PMNs) showed a reduction in Ly6C on day 1 with a trending decrease to MHCII. CD45 trended downward on day 1 and was significantly reduced on day 3. On day 14, population percent increased. On day 28, population percent decreased, but CD45, Ly6C, and MHCII showed significant increases. *p < 0.05, ^†p < 0.10