Fig. 4
Untargeted metabolomic temporal analysis. A Heatmap of top 50 significant metabolites. Limma-based linear modeling, Pearson distance, Ward clustering. B Venn diagram of statistically significant metabolites per day. Day 1 has the strongest overlapping signature with each subsequent day. Overlap by day is as follows, D1:D3 = 13, D1:D7 = 17, D1:D14 = 14, D1:D28 = 40, D3:D7 = 1, D3:D14 = 2, D3:D28 = 4, D7:D14 = 10, D7:D28 = 8, D14:D28 = 6. C Jaccard index correction for multiple statistically significant metabolites. Day 1 has the strongest overlap with day 28 (j-index = 0.1266), while days 7 and 14 have the strongest overlap with each other (j-index = 0.0943). B, C Data normalized by quality control, unlogged, student’s t test. D Volcano plot overlay from each day. Arg Arginine, Asn Asparagine, DHP Dihydroprogesterone, Gln glutamine, MT Methoxytyramine, NAA N-acetyl-L-aspartic acid, FC fold-change, p: p value. Data normalized by quality control samples and log2()