Fig. 5

Nrf2^−/− mice showed impaired bilateral-brain functional connectivity. A Seed-based analysis represented by functional connectivity maps for Nrf2^+/+ and Nrf2^−/− mice. The strength of connectivity for the seed region, indicated above each image, is mapped by a colour scale representing the correlation coefficient value (CC) value (abbreviations: CA3 = field of CA3 in hippocampus). B and C Average interhemispheric and intrahemispheric functional connectivity for ipsilateral and contralateral seeds in Nrf2^+/+ and Nrf2^−/− mice. D Functional connectivity matrices of Nrf2^+/+ mice (left) and Nrf2^−/− mice (right) (postnatal week 12), in which functional correlation (z score) between pairs of regions is represented by a colour scale (abbreviations: ORB = orbital, PL = prelimbic, IL = infralimbic, ACA = anterior cingulate area, RSP = retrosplenial area, Cpu = caudate putamen, NAc = nucleus accumbens, LSN = lateral septal nucleus, MHb = medial habenula, LHb = lateral habenula, dTHA = dorsal nucleus of thalamus, vTHA = ventral medial nucleus of the thalamus, SN = substantia nigra, PRN = pontine reticular nucleus, PPN = pedunculopontine nucleus). E The functional connectivity matrix of Nrf2^+/+ and Nrf2^−/− mice was subjected to a two-sample T test to obtain a statistically significant matrix, where 0 represents P > 0.05, 1 represents P < 0.05, and 2 represents P < 0.01. F Based on the matrix in D and E, the average functional connectivity strength of the hippocampus with all other regions was calculated for Nrf2^+/+ and Nrf2^−/− mice. G As shown in D, functional connectivity was averaged between the hippocampus and the PFC, Cg, Str, Hb, and THA (n = 6/group). H Pearson linear correlation tests for immobility time in the TST and z scores in the hippocampus. Bars represent the mean ± SEM; statistical analysis was performed by using an unpaired two-tailed t test. *P < 0.05, **P < 0.01