Fig. 1
From: GSDMD gene knockout alleviates hyperoxia-induced hippocampal brain injury in neonatal mice
GSDMD expression in the brains of the four study groups. A GSDMD immunostaining showed GSDMD expression was increased in the hippocampal area of hyperoxia-exposed wildtype (WT-O2) brain (red arrows) compared to the hippocampal areas of the room air-exposed WT (WT-RA), room air-exposed GSDMD-KO (KO-RA), and hyperoxia-exposed GSDMD-KO (KO-O2) hippocampi. Representative focal enlarged areas of GSDMD + cells are in the red boxes. 20 × objective magnification. Scale bar: 50 µm. B Double immunofluorescent staining for GSDMD (green signal), AIF1 (red signal), and DAPI (blue signal, not shown). GSDMD was co-localized with AIF1 in WT-O2 hippocampus (orange signals, white arrows). Scale bar: 50 µm. C Double immunofluorescent staining for GSDMD (green signal), GFAP (red signal), and DAPI (blue signal, not shown). GSDMD was co-localized with GFAP in the WT-O2 hippocampus (orange signals, white arrows). Scale bar: 50 µm