Fig. 2
From: GSDMD gene knockout alleviates hyperoxia-induced hippocampal brain injury in neonatal mice
GSDMD-KO reduces hyperoxia-induced hippocampal inflammation. A Immunostaining for AIF1 (a microglial marker, brown signals, red arrows) (A) and immunostaining for CD68 (a microglial marker, brown signals, red arrows) (B) showed microglia cells in the WT-O2 hippocampus were disorganized and had enlarged bodies and dendrites compared to hippocampi from WT-RA, KO-RA, and KO-O2 mice. Representative focal enlarged areas of microglial cells are in the red boxes. There was a significant increase of AIF1 + microglial cells (C) and CD68 + microglial cells (D) in the WT-O2 group compared to the WT-RA group, but KO-O2 had reduced microglial cells compared to the WT-O2 group. n = 5/group. ***P < 0.001 and ****P < 0.0001, WT-RA vs. WT-O2. ###P < 0.001 and ####P < 0.0001, WT-O2 vs. KO-O2. 20 × objective magnification. Scale bars: 50 µm. E qRT-PCR demonstrated increased gene expression of inflammatory mediators, including Il6, Il1r1, Il18, and Il33, in WT-O2 brains compared to WT-RA brains. However, KO-O2 mice had reduced expression of these genes compared to WT-O2 brains. n = 4/groups. *P < 0.05 and ***P < 0.001, WT-RA vs. WT-O2. #P < 0.05, ##P < 0.01 and ###P < 0.001, WT-O2 vs. KO-O2