Fig. 3
From: GSDMD gene knockout alleviates hyperoxia-induced hippocampal brain injury in neonatal mice
GSDMD-KO improves cell survival in hyperoxia-exposed brains. A Representative immunofluorescence staining for Ki67 (pink signals, red arrows) and DAPI nuclear staining (blue signals) in brain tissue sections from WT-RA, WT-O2, KO-RA, and KO-O2 mice. Representative focal enlarged areas of Ki67 + stained cells are in the white boxes. B Quantification of proliferation index (percentage of Ki67 + nuclei/total cell nuclei) showed a decreased Ki67 + cells in the WT-O2 group. In contrast, the GSDMD-KO group exposed to hyperoxia had increased Ki67 + cells compared to the WT-O2 group. n = 5/group. ***P < 0.001, WT-RA vs. WT-O2. #P < 0.05, WT-O2 vs. KO-O2. 20 × objective magnification. Scale bars: 50 µm