Fig. 4
From: GSDMD gene knockout alleviates hyperoxia-induced hippocampal brain injury in neonatal mice
GSDMD-KO prevents cell death induced by hyperoxia. A TUNEL assay (green signals) and DAPI nuclear stain (blue signals) were used to identify dead cell nuclei (teal signals). Representative focal enlarged areas of TUNEL + stained cells were in the white boxes. B Quantification of cell death index (percentage of apoptotic nuclei divided by total nuclei) revealed that WT hippocampus had increased cell death when exposed to hyperoxia. In contrast, hyperoxia-exposed KO hippocampus had significantly less cell death. n = 5/group. **P < 0.01, WT-RA vs. WT-O2. ##P < 0.001, WT-O2 vs. KO-O2. 20 × objective magnification. Scale bars: 50 μm. C TUNEL assay (green signal) was performed with immunofluorescence for SOX2 (red signal), and DAPI (blue signal, not shown). TUNEL-positive nuclei were co-localized in SOX2-positive cells in WT-O2 hippocampus (white/yellow nuclei, white arrows). Scale bar: 50 µm. D TUNEL (green signal) was performed with immunofluorescence for GFAP (red signal), and DAPI (blue signal, not shown), TUNEL-positive nuclei were co-localized in GFAP-positive cells in WT-O2 hippocampus (teal nuclei/red cells, white arrows). Scale bar: 50 µm