Fig. 7
From: GSDMD gene knockout alleviates hyperoxia-induced hippocampal brain injury in neonatal mice
GSDMD KO modulates the transcriptional response to hyperoxia in the hippocampus. A Volcano plot of genes differentially expressed in brains of KO-O2 mice compared to WT-O2 mice (fold change > 1.25 and FDR < 0.1). B Gene set enrichment analysis of genes differentially expressed in hyperoxia-exposed KO mice compared to hyperoxia-exposed WT mice. In the KO-O2 group, there was an induction of genes associated with negative regulation of nervous system development, regulation of neuronal synaptic plasticity, hippocampus development, negative regulation of neurogenesis, endothelial cell chemotaxis, postsynapse organization, extracellular structure organization, and VEGFA/VEGFR2 signaling compared to WT-O2 group. Hyperoxia-suppressed gene pathways were associated with neurotransmitter receptors and postsynaptic signal transmission, neuroactive ligand receptor interaction, synaptic transmission, synapse asseembly, blood vessel diameter maintenance, vascular process in circulatory system, reponse to BMP, and oligodendrocyte differentiation. C Network plot of select top biological processes and their associated genes induced in KO-O2 compared to WT-O2. D Network plot of select top biological processes and their associated genes suppressed in KO-O2 compared to WT-O2 brains. n = 4 animals/group in WT groups. n = 3 animals/group in KO groups