Fig. 2
The expression of excitatory synaptic markers in perilesional M1 and PMC. a Box-and-whisker plots with vertical scatter plots of individual cases showing the density (% area labeled) and average particle size (area in µm2) of VGLUT1 + puncta in perilesional M1 and in PMC of each monkey. b Representative confocal images of VGLUT1 immuno-labeling in M1 and PMC. c Box-and-whisker plots with vertical scatter plots of individual cases of the density and average size of VGLUT2 + puncta in perilesional M1 and in PMC. The density of VGLUT2 + puncta was significantly lower in groups with lesions compared with Ctr in both perilesional M1 (one-way ANOVA, main effect, p < 0.001; Fisher’s LSD post hoc, ctr. vs veh: p = 0.004; ctr vs EV: p = 0.001) and in PMC (one-way ANOVA, main effect, p = 0.05; Fisher’s LSD post hoc, ctr. vs veh: p = 0.04; ctr vs EV: p = 0.02). d Representative maximum-projection confocal images of VGLUT2 immuno-labeling in M1 and PMC. e Box-and-whisker plots with vertical scatter plots of individual cases of the density (% area label) and average size (µm2) of GluR2/3 + puncta in perilesional M1 and in PMC. The density of GLUR2/3 + puncta in PMC was significantly lower in the vehicle group (one-way ANOVA, main effect, p = 0.1; Fisher’s LSD post hoc, p = 0.036) but not in the EV group (Fisher’s LSD post hoc, p = 0.49) as compared with non-lesion controls. f Representative maximum-projection confocal images of GLUR2/3 immuno-labeling in M1 and PMC. For a-f Ctr: n = 3. Veh: n = 4. EV: n = 5 monkeys Scale bar: 20 µm. Box-and-whisker plots: bars show interquartile range and median (horizontal line) with error bars = 95% confidence interval; *p < 0.05, **p < 0.01