Fig. 3
The expression of inhibitory synaptic markers in perilesional M1 and PMC. a Box-and-whisker plots with vertical scatter plots of individual cases showing the particle density and average size of VGAT+ puncta in perilesional M1 and in PMC (Ctr: n = 3; Veh group: n = 5; EV group: n = 5 monkeys). b Representative maximum-projection confocal images of VGAT immuno-label in M1 and PMC. c Box-and-whisker plots with vertical scatter plots of individual cases of the density and average size of GABAa α1+ puncta in perilesional M1 and in PMC. The density of GABAa α1 subunit in perilesional M1 was significantly lower in both veh and EV group as compared with non-lesion controls (one-way ANOVA, main effect, p = 0.004; Fisher’s LSD post hoc, ctr. vs. Veh, p = 0.038; ctr. vs. EV, p = 0.009). The density of GABAa α1 in PMC was lower in veh and EV group (one-way ANOVA, main effect, p = 0.016; Fisher’s LSD post hoc, ctr. vs. veh, p = 0.05; ctr. vs. EV, p = 0.02). The size of GABAa α1 in M1 was significantly smaller in the EV group (p < 0.001). d Representative maximum-projection confocal images of GABAa α1 receptor subunit immuno-label in M1 and PMC. e Box-and-whisker plots with vertical scatter plots of individual cases showing the particle density and average size of GABAb R2+ puncta in perilesional M1 and PMC. f Representative maximum-projection confocal images of GABAb R2 subunit immuno-label in M1 and PMC. For c-f Ctr: n = 3; Veh group: n = 4; EV group: n = 5 monkeys. Scale bar: 20 µm. Box-and-whisker plots: bars show interquartile range and median (horizontal line) with error bars = 95% confidence interval; *p < 0.05, **p < 0.01