Fig. 6
C1q co-expression on VGLUT2 + axon terminals and Iba1 microglia. Box-and-whisker plots with vertical scatter plots of individual cases showing: a The density (% area label) of C1q + puncta in perilesional M1 and PMC (one-way ANOVA, main effect, p = 0.07; Fisher’s LSD post hoc, M1: ctr. vs EV p = 0.028). Ctr: n = 3. Veh: n = 4. EV: n = 5 monkeys. b Fold changes of C1QA and C3 gene expression in perilesional M1 (t-test, veh. vs. EV, p = 0.027; Ctr: n = 3. Veh: n = 4. EV: n = 4). Ctr: n = 3; Veh: n = 4; EV: n = 4 monkeys; and c the density (% area label) of VGLUT2 + puncta colocalized with C1q + puncta (one-way ANOVA, main effect, p = 0.03; Fisher’s LSD post hoc, ctr. vs. veh, p = 0.02). Ctr: n = 3; Veh: n = 4; EV: n = 5 monkeys. d Representative maximum-projection confocal images of C1q + puncta immuno-labeling in M1 and PMC. Scale bar: 20 µm. e Representative maximum-projection confocal images showing dual channel labeling of C1q (magenta) and VGLUT2 (green), with colocalized VGLUT2-C1q points masked in white. f Box-and-whisker plots with vertical scatter plots of individual cases showing the distance between the C1q-VGLUT2 colocalized points and microglia (Iba1)-VGLUT2 colocalized points. Inset shows schematic diagram of how C1q-VGLUT2-Iba1 distance was determined. Ctr: n = 3; Veh: n = 4; EV: n = 5 monkeys. g Linear regression showing increasing C1q expression correlated with decreasing expression of VGLUT2 + (R2 = 0.378, p = 0.034) in M1. h Linear regression showing greater C1q expression correlated with greater VGLUT2–microglia colocalization (R.2 = 0.372, p = 0.035). i Representative maximum-projection confocal images of 9 optical slices showing single/dual/triple channel labeling (left to right) of colocalized VGLUT2 (green), Iba1 (cyan) and C1q (magenta). Box-and-whisker plots: bars show interquartile range and median (horizontal line) with error bars = 95% confidence interval; *p < 0.05