Fig. 6

Chronic SCI alters cellular inflammation in the spinal cord of male and female. A Representative dot plots depict the relative immune cell composition in the lesion area of the spinal cord at 19 months (mon) post-injury. B, C Cell counts normalized by tissue weight showed increased number of CD45^intCD11b⁺ microglia (B) and CD45^hiCD11b⁻ infiltrating myeloid cells (C) in the spinal cord of SCI/F mice. D Representative histogram showed the relative production of ROS in microglia as measured by H2DCFDA (DCF) dye. E Mean fluorescence intensity (MFI) showed higher ROS production levels in SCI/F compared to Sham/F and SCI/M groups. n = 7 per group. *p < 0.05, **p < 0.01 vs. Sham group; #p < 0.05 vs. SCI group. Two-way ANOVA followed by Fisher’s LSD test for multiple comparison (A, E) and by Tukey’s multiple comparisons test (B)