Fig. 7

Neuroinflammation in the brain can be detected long after initial SCI. A, B Flow cytometry quantified the cell counts of CD45^intCD11b⁺ microglia (A) and infiltrating CD45^hiCD11b⁻ myeloid cells (B). C Representative dot plots and percentage of TNF + cells in the microglia. D Representative histogram and MFI of H2DCFDA (DCF) showed increased levels of ROS production in the brain of SCI/F group compared to Sham/F. E Representative histogram and MFI of MitoSpy Red CMXRos showed decreased mitochondrial activity in SCI/M compared to Sham/M while SCI/F showed no difference compared to Sham/F and significantly higher than SCI/M. n = 7 per group. *p < 0.05, **p < 0.01 vs. Sham group; ^#p < 0.05, ^##p < 0.01 vs. SCI group. Two-way ANOVA followed by Tukey’s multiple comparisons test