Fig. 2

Constitutive deletion of C5aR1 significantly alters microbiome of WT and Arc mice when housed separately from their C5aR1-sufficient counterparts. A Experimental overview of separately housed C5aR1 sufficient and deficient animals. B Alpha diversity of fecal samples at 10 months of age by the Shannon Diversity Index when mice with and without C5aR1 are housed separately. C Beta-diversity by Bray–Curtis Dissimilarity of WT and Arctic mice with their corresponding C5aR1KO illustrates the difference in fecal microbiota composition between C5aR1KO and C5aR1-sufficient mice, with corresponding PERMOVA results to the right. Symbol color represents individual cages. D Taxa bar plot of the 6 most abundant orders. E–H The number of amplicon sequence variants (ASV) for the orders Erysipelotrichales (E) and Clostridiales (F) and the genera Alistipes (G) and (H). *p < 0.05; **p < 0.01; ***p < 0.00. ANOVA statistics are available in Additional file 6: Table S1 while beta-diversity PERMANOVA can be found in Additional file 7: Table S2. Additional details are available in Additional file 2