Fig. 4

Treatment with PMX205 does not alter the microbiome of Arctic mice. A Schematic of the Arctic PMX205 study design. B Alpha diversity at 2 months of age, following 2 weeks of treatment with water or PMX205 or 5 months of age following 12 weeks of PMX205. C Beta-diversity, as measured by Bray–Curtis dissimilarities. Beta-diversity is presented as principal component analysis (PCoA) with corresponding PERMANOVA results to right of each graph that the microbiota composition was not altered due to the interaction of genotype and treatment at 2 (C) or 5 (D) months. Stacked taxa bar plots, collapsed to the order level, detailing the top six most abundant orders present following 2 (E) and 12 (F) weeks of treatment. G–J Specific order and genera were analyzed at each age by two-way ANOVA. **p < 0.01; ***p < 0.001 ANOVA statistics are available in Additional file 6: Table S1 while beta-diversity PERMANOVA can be found in Additional file 7: Table S2. Additional details are available in Additional file 4