Fig. 2

IRF-7 limits viral replication in the periphery and the CNS. WT, Irf3^−/− and Irf7^−/− mice were intraperitoneally infected with 10⁴ FFU of LGTV. Viral burden was determined by quantitative RT-PCR in the different brain regions (A) or by plaque assay in the olfactory bulb (B) (n = 10). The dotted line represents the detection limit. Asterisks indicate statistical significance calculated by Student’s t test, *p < 0.05. C Representative pictures of immunohistological analysis of LGTV-E protein in the olfactory bulb, cortex, hippocampus and cerebellum at 7 days post-infection (n = 5) [LGTV E protein (green), DAPI (blue)]. Magnification is represented by scale bar, 200 µm