Fig. 3
IRF7 deficiency increases cytokine response in the brain. WT, Irf3−/− and Irf7−/− mice (n ≥ 10) were intraperitoneal infected with 104 FFU of LGTV. A Level of IFNβ induction in LGTV-infected IFNβ+/Δluc mice. Shown is whole body imaging of Ifnβ+/Δluc WT, Irf3‑/‑ or Irf7‑/‑ reporter mice. Mice were imaged before treatment (0 h) and over time as indicated; one representative mouse is shown in dorsal position. The scale indicates the number of photons (p) measured per s per cm2 per steradian (sr). B Cytokines CCL2 and TNFα were determined by quantitative RT-PCR in the brain. The dotted line represents the detection limit. Asterisks indicate statistical significance calculated by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001