Fig. 6

IRF7 deficiency leads to differences in cellular tropism of LGTV. WT, and Irf7^−/− mice were infected intraperitoneally with 10⁴ FFU of LGTV. Brains were isolated for immunohistological analysis 7 days post-infection. A Representative pictures of IBA-1 (red), NeuN (red), GFAP (red) and, LGTV-E protein (green), and DAPI (blue). Scale bar is shown as 50 μm. B Quantification of LGTV-positive, NeuN⁺, GFAP⁺, and IBA-1⁺ cells in the glomerular layer of the olfactory bulb in infected mice, 7 days post-infection (n = 5). Asterisks indicate statistical significance calculated by Student’s t test, *p < 0.05, **p< 0.01, ***p < 0.001. C Proportion of microglia, neurons and astrocytes from infected cells