Fig. 7

Local antiviral role of IRF7 upon LGTV infection in the brain. WT, and Irf7^−/− mice were infected intracranially with LGTV. A Kaplan–Meier curve of mice infected with 10² FFU (n = 5) or 10 FFU (n = 5). Data are cumulative from two independent experiments. Asterisks indicate statistical significance calculated by Mann–Whitney test, *p < 0.05. B IFN-β and IFN-α determination by quantitative RT-PCR in the brain of mice infected intracranial by 10 FFU with LGTV. Asterisks indicate statistical significance calculated by Mann–Whitney test (A) or Student’s t test (B), *p < 0.05, **p < 0.01. Determination of viral titers (B) and IFN-I production (C) from primary hippocampal neuron or whole-brain astrocytes cultures. D Primary astrocytes were transfected with increasing amounts of poly(I:C). Supernatants were harvested 24 h post-transfection and IFN-I was determined by a bioassay. E Survival analysis of LGTV-infected Ifnβ^−/∆luc and Irf7^−/− Ifnβ^−/∆luc mice. Asterisks indicate statistical significance calculated by Mantel–Cox test (A, E) and 2-way ANOVA (B–D), *p < 0.05, ***p<0.001. Results are representative for at least two independent experiments