Fig. 7

A-G Representative images of reconstructed hippocampal pyramidal spines revealing changes during aging and neuroinflammation. The bright-field Golgi-stained images of reconstructed pyramidal spines (A–F) represent changes in young, adult, and old mice from control and GFAP-IL6 cohorts. The grouped bar graph (G) represents the estimated spine density per µm of reconstructed pyramidal dendrite in the hippocampus of young (n = 20), adult (n = 20) and old (n = 20) mice from control and GFAP-IL6 cohorts. Data presented as mean ± SEM and analysed with two-way ANOVA followed by Tukey’s post hoc tests. Post hoc effects of ‘genotype’ are represented in asterisks (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001); post hoc effects of ‘age’ are represented by hash symbols (^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001). Images taken under the 60× oil-objective (scale bar 100 µm)