Fig. 4

Repopulated B cell subsets increase CD20 expression. A Heatmap indicates CD20 median scaled intensity across B cell subpopulations. The size of the circles (right) represents the size of the p-value and the colour represents the direction of change between the different experimental groups. B Schematic overview of the longitudinal study design and timeline. C Bar plots display the percentage of annotated B cell subsets out of the total CD45⁺ cells from patients that went from SID to SID, SID to EID, EID to SID and EID to EID. Each data point corresponds to each individual, columns and error bars show mean ± SEM. P-values indicate the statistical differences after a GLMM model with age, sex and type of MS as covariates and patient ID as a random effect. D Violin plots display the Δ or subtraction of the percentage of annotated B cell subsets out of the total CD45⁺ cells at the second blood sampling minus the percentage of annotated B cell subsets out of the total CD45⁺ cells at the first blood sampling. P-values indicate the statistical differences after a GLM model of the change of percentages between groups of patients, with age, sex and type of MS as covariates. *adjusted p-value < 0.05, **adjusted p-value < 0.01, ***adjusted p-value < 0.001, ****adjusted p-value < 0.0001; #unadjusted p-value < 0.05. OCR ocrelizumab, GLM multivariate general linear model; GLMM multivariate general linear mixed model, CG control group, SID standard interval dosing, EID extended interval dosing, T1 first-time point/blood sampling, T2 second-time point/blood sampling