Fig. 9

PAD4 promotes neuroinflammation and neuronal death via IRE1α/ASK1/JNK signaling pathway after TBI. A Schematic image of adeno-PAD4-EGFP and overexpression of PAD4 in the cortex of the mouse. B, C mNSS test on day 1 (B) and days 3 (C) post-TBI. **p < 0.01, ***p < 0.001, n = 10 per group. D, E Rotarod test on day 1 (D) and days 3 (E) post-TBI. *p < 0.05, **p < 0.01, ***p < 0.001, n = 10 per group. F Representative western blot bands and densitometric quantification of Iba-1, CD16, and CD206 in the contused cortex after TBI. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6 per group. G Representative image of the FJC (green) staining and representative images of TUNEL (green) co-localization with neurons (NeuN, red) in the contused cortex at 3 days after TBI. Nuclei were stained with DAPI (blue). Scale bar = 100 μm. H, I Quantitative analysis of FJC-positive cells (H) and quantitative analysis of TUNEL-positive neurons (I) in the contused cortex at 3 days after TBI. *p < 0.05, **p < 0.01, n = 6 per group. J Representative western blot bands and densitometric quantification of pIRE1α, pASK1, and pJNK in the contused cortex after TBI. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6 per group