Fig. 6

ShRNA PSMC5 inhibited MyD88-dependent signaling pathway activation and suppressed transition of microglial polarization from M2 to M1 phenotype under neuroinflammatory conditions. Protein expression of signaling components of the MyD88-dependent pathway were analyzed using the indicated antibodies by western blot. n = 3–4 mice/group.Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 compared to the control, saline, shRNA PSMC5, and VIPER groups; ^#P < 0.05, ^##P < 0.01 compared to the shRNA PSMC5 + LPS, VIPER + LPS, and shRNA PSMC5 + VIPER + LPS groups, analyzed by one-way ANOVA. Error bars indicate SEM