Fig. 6

GLUT1 inhibition altered cognitive functions and brain metabolic profiles. A, B Y-maze test on POD 14, including: the number of errors (A) and the latency (B), student’s t test applied to the analysis of latency and Mann–Whitney test was applied to the number of errors with n = 7–8 mice per group. C, D NOR test on POD 14, including: discrimination index of two similar objects (A1 and A2) on POD 13 training (C); discrimination index of novel object (B) on POD 14 NOR testing (D), student’s t test with n = 7–8 mice per group. E Heatmap of metabolites in the postsurgical hippocampus on POD 14. F: PLSDA score plot. G VIP scores of PLSDA model. Data was presented as mean ± SEM, *P < 0.05, **P < 0.01. LAP, laparotomy; GLUT1,  GLUT1 overexpression by AAV9-ICAM2-GLUT1; STF-31, GLUT1 specific inhibitor; PLSDA, Partial least squares discriminant analysis; VIP scores, variable importance scores