Fig. 7

RNA-sequencing confirms the role of Ripk2-deficient microglia in ischemic stroke injury. A Flow cytometric gating strategy for microglia. B Principal component analysis (PCA) plot of transcriptomes of microglia isolated from WT and Ripk2 KO mice following ischemic stroke. C Volcano plot of differentially expressed genes (DEGs, fold change > 1.5, FDR < 0.05) between WT and KO mice. The number of genes enriched in the indicated groups is shown in parentheses. D DAVID Gene Ontology (GO) analysis for biological process using upregulated DEGs in KO group. No GO terms (FDR < 0.1) were found when using enriched DEGs in the WT group. E Heat map of DEGs related to pathways shown in C. n = 4/genotype. To correct for differences in infarct volume, animals were subjected to different times of occlusion (45 min for WT, 60 min for Ripk2 KO). Microglia were isolated 24 h post-stroke