Fig. 1
From: Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity
Knockdown of NFIA attenuates 4-AP-induced seizure and neuronal damage. A Scheme of in vivo and in vitro experiments in 4-AP-induced seizure mouse model. Behavioral evaluation showing 4-AP-induced seizure grade (Bi), seizure latency (Bii) and seizure duration (Biii) in mice with hippocampal si.NC or si.NFIA delivery (n = 12 for each group, ****P < 0.0001, Student’s t test). Representative EEG recordings (C) in mice with ip. 4-AP or saline (n = 6 for each group). D HE and Nissl E staining in the hippocampal tissues from the mice with si.NC/si.NFIA delivery and ip. 4-AP/saline, especially showing darker staining in DG and hilus, with cytoplasmic shrinkage, and triangulated pyknotic nuclei in si.NC group, but not in the si.NFIA group (n = 6 for each group, ***P < 0.001, one-way ANOVA)