Fig. 3
From: Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity
4-AP-induced NFIA upregulation is cell-specific in mouse hippocampal astrocytes. Representative confocal fluorescent images of NFIA+ astrocytes (Ai) in hippocampal tissues and quantitative analysis of NFIA+ astrocytes in CA1, CA2, CA3 and DG (Aii) from the mice with ip. 4-AP or saline (n = 6 for each group, *P < 0.05, Student’s t test). B Scheme of primary astrocyte, microglia, neuron culture and 4-AP exposure for protein analysis. Representative immunoblot bands (C) and protein level analysis (D) of NFIA in three types of primary cultured cells treated with 4-AP or saline (n = 3 for each group, *P < 0.05, Student’s t test)